Regulatory

Part:BBa_K187012

Designed by: Team Biobytes   Group: iGEM09_Alberta   (2009-10-17)


Sigma 70 50% promoter in pBA, BioBytes plasmid

The 50% promoter produces ~50% of the expression level from a consensus promoter. The pBA plasmid (part BBa_K187000) adapts the promoter for assembly with other parts using the BioBytes assembly system. See the BioBytes BBF RFC for details on this method.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 47
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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