Composite

Part:BBa_K1869002

Designed by: Conary Meyer   Group: iGEM15_Santa_Clara   (2015-09-18)


Inducible CFA Synthase

This is a composite part of BBa_R0010, BBa_B0034, and BBa_K1869000 designed to allow for IPTG inducible transcription of Cyclopropane Fatty Acid (CFA) Synthase. This composite part is intended to demonstrate the effect of the CFA synthase in vivo. It can be used to increase the acid resistance of the transformed organism by changing the phospholipid profile to include CFAs which decreases the membrane's permeability to weak acids.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 476
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 473


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Categories
Parameters
None