Part:BBa_K1869000
CFA Synthase
The cyclopropane fatty acid (CFA) synthase is a cytosolic protein that reversibly associates with the membrane and catalyzes the addition of a methyl group. The methyl group is taken from S-adenosylmethionine (SAM) and attached to monounsaturated fatty acids (UFA) to generate CFAs. This part is useful in fortifying an organism's membrane. By expressing this protein, the phospholipid profile of the membrane changes to include these CFAs which decreases its permeability to small molecules, specifically weak acids. By decreasing its permeability, it is possible to increase the acid resistance of the cell to allow it to survive in more acidic conditions. This part is useful for creating organisms that are more tolerant of prolonged cultures or creating probiotics capable of surviving in the gut.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 250
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 295
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 247
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