Reporter

Part:BBa_K1865001

Designed by: Avril von Hoyningen-Huene, Sindhu Thiagarajan, Verena Siebert   Group: iGEM15_Goettingen   (2015-09-17)


eforRed_BCEL

eforRed_BCEL consists of the dockerin BCEL (BBa_K1865000) and eforRed (BBa_K592012). By adding our dockerin to this BioBrick from the Uppsala 2011 team, we have made it compatible for use with our scaffoldin. The eforRed that will bind to the equivalent cohesin on a scaffoldin via the dockerin connected to it. The dockerin is derived from Bacteroides cellulolyticus and binds to the cohesin consensus sequence from this organism. eforRed was codon optimized for use in E.coli when used by Uppsala in 2011. It originally stems from

eforRed_BCEL can used as a reporter for the detection of the scaffoldin construct in a sample.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None