Composite

Part:BBa_K1861070

Designed by: Vladyslav Vyshnevskyi   Group: iGEM15_FAU_Erlangen   (2015-09-18)

Fusion protein of TALE3 and a SpyTag in an expression cassette

This part includes the TALE BBa_K1861701 fused N-terminally with the SpyTag BBa_K1159201 and cloned into our yeast expression cassette BBa_K1861010.
The TALE has the repeat variable diresidues HD HD NH NI HD NG HD NH HD NG NH NG and binds the sequence T CCGACTCGCTGT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1364
    Illegal NheI site found at 2445
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
    Illegal XhoI site found at 2241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 707
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
//function/regulation/transcriptional
//proteindomain/dnabinding
Parameters
None