Generator

Part:BBa_K1859024

Designed by: Yusuke Banno   Group: iGEM15_Gifu   (2015-09-10)

mRNA circularize efficiently generator (inside complementarityⅠ)

We designed this generator [BBa_K1859024] to improve circular efficiency of mRNA including His-RFP sequence.

The generator is comprised of Circular parts combined the linker( [BBa_K1859003] and [BBa_K1859004] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .

Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004] . RNA sequences of these linkers bind complementary with each other in circular mRNA.

We found that circular efficiency of [BBa_K1859024] was the third, when we compared with amount of circular mRNA made by these generators [BBa_K1332011] , [BBa_1859024], [BBa_K1859025] , [BBa_K1859026] .

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