Project

Part:BBa_K1857002

Designed by: Joshua Timmons   Group: iGEM15_Northeastern_Boston   (2015-09-16)


HSP70A-RBCS2_Hyg Expression Plasmid

This plasmid is designed to facilitate the easiest expression of proteins possible. It contains an HSP70A-RBCS2 fusion promoter attached to an RBCS2 intron, flanked by the iGEM prefix and suffix. There is an RBCS2 3' UTR after the suffix. Additionally, there is a complete Hygromycin B resistance cassette downstream, allowing for selection.

The suffix can be removed and replaced with the coding sequence of a heterologous protein. Furthermore, the entire fusion-promoter-intron sequence can be removed with the prefix and suffix and replaced by an alternate promoter to compare expression levels between Chlamydomonas promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


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