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Part:BBa_K1857001

Designed by: Joshua Timmons   Group: iGEM15_Northeastern_Boston   (2015-09-16)


BBa_K1857001

This resistance cassette confers resistance to Hygromycin B. Its resistance gene, Aph7", is under an HSP70A-RBCS2 fusion promoter, with an RBCS2 intron for greater expression, and before an RBCS2 3' UTR.

Berthold P, Schmitt R, Mages W (2002) An engineered Streptomyces hygroscopicus aph 7″ gene mediates dominant resistance against hygromycin B in Chlamydomonas reinhardtii. Protist 153:401-12


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1109
    Illegal NgoMIV site found at 1291
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1564


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