Coding

Part:BBa_K1855004

Designed by: Iain Galpin   Group: iGEM15_Warwick   (2015-09-17)


INP-Zif268

A fusion protein of a transmembrane domain (INP) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1047
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1008
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//proteindomain/dnabinding
//proteindomain/transmembrane
Parameters
None