Device

Part:BBa_K185053

Designed by: Shikun Zhao   Group: iGEM09_SJTU-BioX-Shanghai   (2009-10-18)


Promoter116+RBS34+LacI+Double terminator+Plac+RBS31+RelE+RBS30+Lon protease+Double terminator

This device composes of promoter, RBS, LacI, Plac, RelE, Lon and so on, which can express relE and Lon protein. The protein expression is controled by lacI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 2474
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3187
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2474
    Illegal AgeI site found at 3650
    Illegal AgeI site found at 3734
    Illegal AgeI site found at 3932
    Illegal AgeI site found at 3965
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None