Composite

Part:BBa_K185051

Designed by: Heng Luo   Group: iGEM09_SJTU-BioX-Shanghai   (2009-10-17)

promoter(constitutive)+rbs+SmpB+double terminator+pBAD+tmRNA

This part is the entire E. coli rescue system in our design.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1926
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1761
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1743


[edit]
Categories
Parameters
None