Intermediate

Part:BBa_K185017

Designed by: Shikun Zhao   Group: iGEM09_SJTU-BioX-Shanghai   (2009-10-16)

Promoter118+LacI+RBS34

This part is an intermediate of BBa_K185016. If you add a double terminator at the end of this part you can express the LacI protein. However the promoter118(BBa_J23118) inside this part lack of a "T", so we do not comfirm this part have function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None