Regulatory

Part:BBa_K1848006

Designed by: Samuel Magaziner   Group: iGEM15_Columbia_NYC   (2015-09-18)


LuxR/AHL driven Lysis Cassette with Protein level Timer Delay

This system presents an AHL/LuxR dependent mechanism for delayed cell lysis. Following induction with AHL, the repressible pLux becomes inhibited and ceases producing repressor protein cI. Following this the pcI becomes uninhibited and proceeds to translate regions encoding lysis inducing proteins. Taken together with the need for AHL/LuxR complexation and cI's ceased production, this exemplifies a time delayed quorum sensing dependent kill switch for the cell. Note: this part does not include the LuxR coding region. Without this within the plasmid as well, the system will not function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2246
    Illegal NheI site found at 2269
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1851
    Illegal AgeI site found at 1921
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2502


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Categories
Parameters
None