Part:BBa_K1847000

promoter-RBS- annexin AV

Annexin V is member of the annexin family of Ca⁺² dependent proteins. Its function is not yet known. It binds to phosphatidylserine in a Ca⁺²-dependent fashion¹. It is widely used for detection of apoptosis, as it binds with 0.5-1 nM affinity to phosphatidylserine, a phospholipid usually found in the inner membrane of the cell, which flips to the outer membrane in the event of apoptosis ^2,3.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

J23114-B0034-Annexin V was cloned into a high copy backbone (pSB1C3) and a low copy backbone (pSEVA371). As can be seen in the Western blot image below, annexing V does no have any detectable levels of expression with pSEVA371 but it is expressed with pSB1C3.

When RBS BBa_B0032 was used instead of BBa_B0034 no expression of Annexin V was observed in the high copy number plasmid.

References

1. Bouter A. Placenta 2015 vol. 36, Supplement 1, S43–S49
2. Pietkiewicz S. et al. J Immunol Meth 2015 vol. 423, 99–103
3. Tait J.F. J Nucl Med October 2008 vol. 49 no. 10 1573-1576

Functional Parameters

dissociation_constant	0.5-1 nM
family	Annexins