Part:BBa_K1846008

Bacteriophage lambda tip attachment protein J

Sequence for the bacteriophage lambda J gene.

Usage and Biology

The bacteriophage lambda J gene encodes the tip attachment protein that forms the base plate of the bacteriophage's tail. It is responsible for host protein recognition and specificity. C-terminal residues of protein J have direct binding interactions with the maltoporin (LamB), a membrane protein of E.coli, allowing irreversible attachment of the phage to the host and facilitating DNA injection [1],[2].

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 577
  Illegal PstI site found at 727
  Illegal PstI site found at 1886
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 577
  Illegal PstI site found at 727
  Illegal PstI site found at 1886
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 577
  Illegal PstI site found at 727
  Illegal PstI site found at 1886
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 577
  Illegal PstI site found at 727
  Illegal PstI site found at 1886
  Illegal AgeI site found at 1471
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Chatterjee, S., & Rothenberg, E. (2012). Interaction of bacteriophage λ with its E. coli receptor, LamB. Viruses, 4(11), 3162–3178. doi:10.3390/v4113162

[2] Werts, C., Michel, V., Hofnung, M., & Charbit, A. (1994). Adsorption of bacteriophage lambda on the LamB protein of Escherichia coli K-12: point mutations in gene J of lambda responsible for extended host range. Journal of Bacteriology, 176(4), 941–947.