Reporter

Part:BBa_K1845000

Designed by: Edoardo Gianni   Group: iGEM15_London_Biohackspace   (2015-09-18)


RFP Reporter/Screening plasmid intermediate

This part is an improvement on BBa_I13507, which has been distributed with the incorrect sequence.

It consists in a Reporter Coding Regions of an RBS (B0034) an mRFP coding sequence (E1010), a barcode, and B0015 terminator. It can be used downstream of a promoter to test it's efficacy. We hope that this part will be used in place of the incorrect twin BBa_I13507

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None