Part:BBa_K1844002

Pta Coding Region

This part includes the coding sequence of the Phosphate acetyltransferase gene of Synechocystis PCC 6803, which catalyzes the reaction converting Acetyl CoA to acetyl-phosphate in the biomass formation pathway of Synechocystis. Overexpressing this gene in Synechocystis has been shown to result in increased acetate production in Synechocystis, suggesting that pta encodes the enzyme with flux control in the acetate production pathway. We've shown that over-expression of pta imposes a growth disadvantage compared to strains without over-expression though, suggesting that it will eventually be filtered out of the population by a mutant. We recommend to combine the coding region of pta with a light-inducible repressor for constitutive expression (if used in Synechocystis) under dark conditions in which growth does not occur and evolutionary pressure will not select for those with highest growth, which would ultimately result in the demise of producing strains.

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SDS-page and inducible promoter results will follow shortly.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1213
  Illegal NotI site found at 1207
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1933
  
• 1000
  COMPATIBLE WITH RFC[1000]