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Part:BBa_K1844000

Designed by: David Lips   Group: iGEM15_Amsterdam   (2015-09-17)


acs KO construct for Synechocystis

This part can be used to knock out the native Acetyl-CoA Synthase (acs) gene of Synechocystis PCC. 6803 via homologous recombination, which induces growth-coupled acetate production.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 784
    Illegal SpeI site found at 1144
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 784
    Illegal SpeI site found at 1144
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 784
    Illegal SpeI site found at 1144
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 784
    Illegal SpeI site found at 1144
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None