Part:BBa_K1843000
Thrombin switch 1.0
Switch 1.0 (adapted for thrombin) with additional cut sites and a GFP reporter was submitted as a part. This part has been demonstrated as an RNA toehold switch, which responds to a specific DNA trigger and expresses GFP, with low background, as seen in Figure 1 of the results (LINK) section. This provides a starting point for other teams looking to adapt toehold switches.
Additionally, the part submitted gives the many other teams inspired by the same publications, an easy control for in vitro translation reactions. Since it already contains a full switch and reporter, it can be transformed and purified and then used directly with a cell-free expression system, without any further cloning. The trigger is short enough to be quickly and cheaply bought as a DNA oligo. Thus, future teams exploring cell-free expression systems can test their systems induced with a DNA oligo (positive reaction control) and without (negative reaction control).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 25
Illegal XbaI site found at 31
Illegal SpeI site found at 972
Illegal PstI site found at 838
Illegal PstI site found at 978 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 25
Illegal SpeI site found at 972
Illegal PstI site found at 838
Illegal PstI site found at 978
Illegal NotI site found at 37 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 25
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 25
Illegal XbaI site found at 31
Illegal SpeI site found at 972
Illegal PstI site found at 838
Illegal PstI site found at 978 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 25
Illegal XbaI site found at 31
Illegal SpeI site found at 972
Illegal PstI site found at 838
Illegal PstI site found at 978
Illegal AgeI site found at 189
Illegal AgeI site found at 312
Illegal AgeI site found at 966 - 1000COMPATIBLE WITH RFC[1000]
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