Coding

Part:BBa_K1842004

Designed by: Walter Thavarajah   Group: iGEM15_UC_San_Diego   (2015-09-17)


LuxDEC

The part codes for the fatty acid reductase complex of the bacterial lux system from Photobacterium Phosphoreum. The system recruits fatty acids and converts them into aldehyde to be used as substrate in the system's light producing reaction. The genes coding for each subunit of the complex are separated by a P2A sequence for stoichiometric expression. The part is comprised of three unique genes - LuxC, LuxD, and LuxE, coding for a reductase, transferase, and synthetase, respectively. Each gene has an attached antibody tag - LuxC has a FLAG tag, LuxD has a V5 tag, and LuxE has a HA tag. One copy of each gene is present. Genes used are codon optimized for expression in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1289
    Illegal BglII site found at 2845
    Illegal BamHI site found at 2046
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1723
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2334


[edit]
Categories
Parameters
None