Coding

Part:BBa_K1842000

Designed by: Walter Thavarajah   Group: iGEM15_UC_San_Diego   (2015-08-17)

LuxABfrp

The part codes for a synthetic luciferase and a NADPH-flavin oxidoreductase, joined by a 2A peptide sequence. When provided with exogenous substrate or coexpressed with the lux system fatty acid reductase complex, the part allows for light production. Luciferase catalyzes the conversion of substrate for light production, while the oxidoreductase regenerates FMNH2 for use by luciferase. The 2A peptide allows for stoichiometric expression of the two proteins. The part is comprised of two unique genes - monomeric LuxAB and frp. Each gene has an attached antibody tag - LuxAB has a MYC tag and frp has a HIS tag. Genes used are codon optimized for expression in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1592
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 955
    Illegal AgeI site found at 1549
    Illegal AgeI site found at 2854
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None