Part:BBa_K1841002

plac promoter for lactobacillus casei

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 93
  

The promoter region contains a cre element (catabolite responsive element) overlapping the –35 region, which is followed by a highly conserved sequence, the ribonucleic antiterminator (RAT) sequence,and a terminator structure. In fact, it has been shown that the expression of the lac operon in L. casei ATCC393 [pLZ15–] is subject to dual regulation:

Carbon catabolite repression (CR) and induction by lactose through transcriptional antitermination. This induction mechanism isremarkably different from the system found in the lac operon from L. lactis , where gene expression is controlled by the repressor LacR, with tagatose-6-phosphate as the likely inducer. The above-mentioned CR of the lac operon is mediated by the general regulatory protein, CcpA, possibly by binding to the cre element at the lactose promoter (plac). Furthermore, the antiterminator activity of LacT is also negatively controlled by glucose, possibly by PTS-mediate dphosphorylation. Antiterminator proteins of the BglG family, such as LacT, share three common domains: an RNA binding domain and two PTS regulated domains (PRD-I and PRD-II).

The sequence ATAAAACGTTTACA of the -35 is the cre-like element that depressed by CcpA. The sequence TGTGGATTGTGACTATTTAATTAGGCGAGACCACA of -10 is ribonucleic antiterminator (RAT) region controlled by lacT.

This promoter can be used in lactobacillus casei under the lactose environment.