Part:BBa_K1840006
Device: TT-kgu-mCherry
This device acts as a detector for glucose (and certain derivatives of it). The double transcription terminator ensures, that only the gene downstream of kgu is influenced by the promotor kgu. The promotor (BBa_K1840000) is contained in the genome of Pseudomonas putida and is situated in front of kgu enzymes (2-ketogluconate epimerase, kinase, transporter and reductase). The repressor PtxS can bind the kgu promotor sequence and thus prevent the transcription of the kgu gene. In case the glucose derivative 2-ketogluconate is present, it binds the promotor and leads to a subsequent conformational change. Hence, the repressor can no longer bind and the gene downstream can be transcribed. In our device, the downstream gene is mCherry, codon optimized for Pseudomonas (BBa_K1840004). Therefore, in the presence of glucose, mCherry is expressed. In various experiments, we could see that this device is in deed working.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 146
- 1000COMPATIBLE WITH RFC[1000]
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