Part:BBa_K1840001
Kgu promotor from Pseudomonas putida
This basic part is the promotor in front of four so called kgu genes. They all are associated with 2-ketogluconate (2-ketogluconate epimerase, kinase, transporter and reductase) in Pseudomonas putida. All of them are concerned with the glucose metabolism in P. putida. The kgu promotor is negatively regulated by the repressor called PtxS. The glucose derivative 2-Ketogluconate can bind to it and this leads to subsequent release of PtxS from the promotor. For our project (NTNU Trondheim 2015) we integrated the promotor in front of the mCherry gene. Hence we built a glucose detection device, that expresses mCherry according to the glucose (and therefore 2-Ketogluconate) level in the environment. Note: The promotor gad (BBa_K1840003) is repressed by the same repressor PtxS. See biobrick parts BBa_K1840000 and BBa_K1840002 for other promotors on operons regulated by glucose derivatives.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 43
- 1000COMPATIBLE WITH RFC[1000]
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