Part:BBa_K1838007

MLC Glucose Sensitive Yellow Chromoprotein Construct

This composite part features our MLC2 construct combined via 3A assembly with a yellow chromoprotein+RBS part (BBa_K1033931). During transcription, the MLC2 binding site is bound by the protein MLC, inhibiting production of yellow chromoprotein. As glucose concentration increases in the cell, more MLC is uptaken and bound to the membrane by a mechanism involving the transmembrane glucose permease called IICBGlu. This means that higher glucose concentrations subsequently result in higher expression levels of the yellow chromoprotein and thus makes it a glucose-sensitive chromoprotein producing part. Specifically, this part tests the hypothesis for how MLC affects the inducibility of an average strength promoter when placed downstream of the base promoter sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]