Regulatory

Part:BBa_K1838001

Designed by: Connor McFadden   Group: iGEM15_UNC-Chapel_Hill   (2015-09-18)


Glucose Sensitive Promoter via MLC E. coli interactions

This part contains the palindromic binding site for the protein MLC encoded by the gene dgsA, a gene native to E. coli. Specifically, MLC is a repressor regulator for many phosphoenolpyruvate-dependent carbohydrate phosphotransferase systems (PTSs). In this part, the binding site is found downstream of the base promoter used for construction, BBa_J23119.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None