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Part:BBa_K1835000

Designed by: Anthony Ciesla   Group: iGEM15_Duke   (2015-09-17)


Empty gRNA Scaffold

Using the restriction enzyme BsaI, an opening will be made to insert annealed oligonucleotides that can code your gRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 81
    Illegal BsaI.rc site found at 58


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