Part:BBa_K1834847
Xylene Sensor! With AmilCP!
Constantly produces XylR and fluoresces in the presence of xylene. The Pu Promoter is activated when xylene becomes attached to XylR, which then produces AmilCP. For additional information on this BioBrick and its functions visit the Consort Alberta 2015 iGEM High School team at: http://2015.igem.org/Team:Consort_Alberta/project
Our experimental trials lead to positive results of this BioBrick part. Using the results we have found, this BioBrick is capable of measuring at least 100 PPM of xylene in a soil sample. When using experimental trials of xylene in LB Broth containing cells, we were able to detect 50 PPM. Further documentation is needed of this part to determine the extent to what amount of xylene this BioBrick can detect.
NOTE: Be prepared if using JM109 cells, as if put under stress, they can turn blue and lead to false results.
We recommend that the incubation of the bacteria last approximately 24 hours for optimal results.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2196 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 147
Illegal BglII site found at 1231
Illegal BglII site found at 1609 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 998
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 851
The sequence of ECOS + AmilCP is as follows: GAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTC CTAGGTACAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGAT GAGCTTGATCTATAAGCCGAAAATGCAGCACGAAGATATGCAGGA TCTGATCTCGCAAATTCGTTTTGTAGCGGCGGAAGGTAAGATCTG GCTTGGCGAACAGCGCATGCTGCTCATGCAGCTGTCCACACTGGC ATCGTTTCGCCGTGAAATTATTAGCTTAATTGGTATTGAACGTGC CAAGGGATTTTTCCTGCGTCTGGGTTATCAGAGCGGATTGATGGA CGCAGAGCTGGCTCGCAAACTGCGTCCGGCGATGCGTGAGGAAGA GGTTTTCTTAGCGGGCCCTCAATTGTACGCGTTAAAAGGCATGGT TAAAGTACGTCTGCTGACAATGGACATCGCGATTCGCGATGGCCG CTTCAACGTGGAGGCCGAATGGATTGATTCGTTTGAGGTGGATAT TTGCCGCACTGAGCTGGGTCTTATGAACGAGCCTGTCTGTTGGAC CGTTCTGGGTTACGCATCAGGGTATGGGAGTGCGTTTATGGGCCG CCGCATCATCTTTCAAGAAACTTCTTGTCGTGGTTGTGGCGATGA TAAATGCCTGATCGTGGGCAAAACCGCGGAGGAGTGGGGGGATGT GAGTTCCTTTGAAGCCTATTTTAAATCCGACCCCATTGTTGACGA GCGCTATGAACTGCAAACCCAGGTGGCTAATCTGCGCAATCGTTT AAAACAGTACGATGGCCAATATTACGGAATTGGGCACTCACCGGC GTACAAACGCATTTGTGAAACCATTGATAAAGCCGCTCGTGGTAA AGTTAGCGTGCTGTTGCTGGGTGAGACCGGAGTAGGAAAAGAAGT GATTGCGCGTAGTGTGCATTTACGTAGCGAACGTGCGGAACAGCC GTTTGTTGCGGTCAACTGCGCGGCCATTCCTCCGGATCTGATTGA AAGTGAACTGTTTGGTGTGGATAAAGGGGCCTATACCGGTGCGGT CAATGCTCGTGCGGGCCGCTTTGAGCGCGCCAACGGTGGTACCAT TTTCCTGGATGAAGTTATTGAACTCACCCCGCGCGCCCAAGCCAC CCTTCTGCGTGTTCTCCAGGAAGGAGAGCTGGAACGTGTTGGTGG TGATCGCACCCGTAAGGTTGATGTGCGTCTGATCACGGCAACCAA CGAGAATCTGGAAGAAGCGGTTAAAATGGGGCGCTTTCGTGCAGA TCTGTTTTTCCGCTTAAACGTCTTCCCGGTCCAAATCCCGCCACT GCGTGAACGCGTGGAAGACATTCCCTTACTGGTGGAACATTTTTT ACGCCGTCATCACAAAGAGTATGGTAAGAAAACGTTAGGTTTATC AGATCGCGCAATGGAAGCGTGCCTGCACTATCAGTGGCCTGGGAA CATCCGCGAGCTTGAAAATGCGCTGGAACGTGGGGTGATCCTGAC CGAATCAAATGAAAGCATTAACGTTGAATCCCTCTTTCCCGGCCT GGCAATGGCAACTGAAGGGGACCGCCTTTCCTCGGAAGGGCGTCT GGAAGAGGAATCTGGTGATTCATGGTTTCGCCAGATTATTGATCA GGGTGTCTCATTAGAAGATCTGGAGGCAGGTCTGATGCGTACAGC GATGGATCGCTGCGGCCAAAACATTTCTCAGGCGGCGCGCCTTCT CGGCCTTACGCGCCCGGCGATGGCATACCGCCTGAAAAAACTTGA TCCGTCGCTGAGCGTGAAAGCCACTGGCCGCTGATAATAATACTA GAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGG CCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGA GTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATAC TAGAGCCCGGGAAAGCGCGATGAACCTTTTTTATCGCTGCCTTGA TCAAATCGACAGGTGGTTATGCGCGATTGATGATTTGCTCAAATA CAGCCAGCGTGCTGTAGATTTTCTCTCATACCCCCCCTTTCTTTT TTACAAAGAAAATCAATAATTTAGATGAAATAAGGGGATCGGTAT AAGCAATGGCATGGCGGTTGCTAGCTATACGAGTACTAGatgagtgt gatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccct acgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcatacc attcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtg tgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcaga agaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcgg tcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcac aacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa
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