Part:BBa_K1834845

Xylene Sensor!

Constantly producing XylR and when in the presence of xylene, the Pu promoter is activated. This BioBrick is also known as ECOS (Environmental COntaminant Sensor). For additional information on this BioBrick and its functions, visit the Consort Alberta High School iGEM team at http://2015.igem.org/Team:Consort_Alberta/project

NOTE: This BioBrick detects XylR, but there is no reporter gene on this version to achieve visual results. See K1834847 for a reporting detector.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 2196
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 147
  Illegal BglII site found at 1231
  Illegal BglII site found at 1609
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 998
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 851
  

The sequence of ECOS is as follows: GAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTC CTAGGTACAGTGCTAGCTACTAGAGAAAGAGGAGAAATACTAGAT GAGCTTGATCTATAAGCCGAAAATGCAGCACGAAGATATGCAGGA TCTGATCTCGCAAATTCGTTTTGTAGCGGCGGAAGGTAAGATCTG GCTTGGCGAACAGCGCATGCTGCTCATGCAGCTGTCCACACTGGC ATCGTTTCGCCGTGAAATTATTAGCTTAATTGGTATTGAACGTGC CAAGGGATTTTTCCTGCGTCTGGGTTATCAGAGCGGATTGATGGA CGCAGAGCTGGCTCGCAAACTGCGTCCGGCGATGCGTGAGGAAGA GGTTTTCTTAGCGGGCCCTCAATTGTACGCGTTAAAAGGCATGGT TAAAGTACGTCTGCTGACAATGGACATCGCGATTCGCGATGGCCG CTTCAACGTGGAGGCCGAATGGATTGATTCGTTTGAGGTGGATAT TTGCCGCACTGAGCTGGGTCTTATGAACGAGCCTGTCTGTTGGAC CGTTCTGGGTTACGCATCAGGGTATGGGAGTGCGTTTATGGGCCG CCGCATCATCTTTCAAGAAACTTCTTGTCGTGGTTGTGGCGATGA TAAATGCCTGATCGTGGGCAAAACCGCGGAGGAGTGGGGGGATGT GAGTTCCTTTGAAGCCTATTTTAAATCCGACCCCATTGTTGACGA GCGCTATGAACTGCAAACCCAGGTGGCTAATCTGCGCAATCGTTT AAAACAGTACGATGGCCAATATTACGGAATTGGGCACTCACCGGC GTACAAACGCATTTGTGAAACCATTGATAAAGCCGCTCGTGGTAA AGTTAGCGTGCTGTTGCTGGGTGAGACCGGAGTAGGAAAAGAAGT GATTGCGCGTAGTGTGCATTTACGTAGCGAACGTGCGGAACAGCC GTTTGTTGCGGTCAACTGCGCGGCCATTCCTCCGGATCTGATTGA AAGTGAACTGTTTGGTGTGGATAAAGGGGCCTATACCGGTGCGGT CAATGCTCGTGCGGGCCGCTTTGAGCGCGCCAACGGTGGTACCAT TTTCCTGGATGAAGTTATTGAACTCACCCCGCGCGCCCAAGCCAC CCTTCTGCGTGTTCTCCAGGAAGGAGAGCTGGAACGTGTTGGTGG TGATCGCACCCGTAAGGTTGATGTGCGTCTGATCACGGCAACCAA CGAGAATCTGGAAGAAGCGGTTAAAATGGGGCGCTTTCGTGCAGA TCTGTTTTTCCGCTTAAACGTCTTCCCGGTCCAAATCCCGCCACT GCGTGAACGCGTGGAAGACATTCCCTTACTGGTGGAACATTTTTT ACGCCGTCATCACAAAGAGTATGGTAAGAAAACGTTAGGTTTATC AGATCGCGCAATGGAAGCGTGCCTGCACTATCAGTGGCCTGGGAA CATCCGCGAGCTTGAAAATGCGCTGGAACGTGGGGTGATCCTGAC CGAATCAAATGAAAGCATTAACGTTGAATCCCTCTTTCCCGGCCT GGCAATGGCAACTGAAGGGGACCGCCTTTCCTCGGAAGGGCGTCT GGAAGAGGAATCTGGTGATTCATGGTTTCGCCAGATTATTGATCA GGGTGTCTCATTAGAAGATCTGGAGGCAGGTCTGATGCGTACAGC GATGGATCGCTGCGGCCAAAACATTTCTCAGGCGGCGCGCCTTCT CGGCCTTACGCGCCCGGCGATGGCATACCGCCTGAAAAAACTTGA TCCGTCGCTGAGCGTGAAAGCCACTGGCCGCTGATAATAATACTA GAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGG CCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGA GTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATAC TAGAGCCCGGGAAAGCGCGATGAACCTTTTTTATCGCTGCCTTGA TCAAATCGACAGGTGGTTATGCGCGATTGATGATTTGCTCAAATA CAGCCAGCGTGCTGTAGATTTTCTCTCATACCCCCCCTTTCTTTT TTACAAAGAAAATCAATAATTTAGATGAAATAAGGGGATCGGTAT AAGCAATGGCATGGCGGTTGCTAGCTATACGAG