Generator

Part:BBa_K1834000

Designed by: Gerry Bourassa   Group: iGEM15_Consort_Alberta   (2015-09-08)


Xylene Sensor!

1. J23100 - which is a constituent promoter. 2. B0034 - the RBS for our XylR gene. 3. I723017 - the XylR coding region which encodes for the transcriptional regulator XylR protein. 4. B0015 - the double stop codon for this sequence. 5. I723020 - This is the Pu promoter. 6. B0030 - RBS.1 strong. Detects xylene in the environment.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 704
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 527
    Illegal NheI site found at 2102
    Illegal PstI site found at 704
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
    Illegal BglII site found at 1609
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 704
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 704
    Illegal NgoMIV site found at 313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 800


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Categories
Parameters
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