Protein_Domain

Part:BBa_K1833012

Designed by: Konstantin Borisov   Group: iGEM15_Pitt   (2015-09-18)


lac promoter -> scFv against Human VEGF-A

This part contains the lac promoter, rbs, and a coding region that encodes for a single chain variable fragment antibody specific against VEGF-A. However, this part does not contain a stop codon or a terminator, because this part was used as a PCR template for further cloning.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 105


[edit]
Categories
Parameters
None