Generator

Part:BBa_K1833008

Designed by: Konstantin Borisov   Group: iGEM15_Pitt   (2015-09-17)

pT7-MMP9 sensitive repressor

This part produces a synthetic repressor in response to T7 RNA Polymerase. This synthetic repressor consists of a fusion of two DNA binding domains connected with a linker that is sensitive to matrix metalloprotease 9, and it represses the promoter described by parts BBa_K1833001 and BBa_K1833003. For more information on the use of this part, visit the 2015 Pitt iGEM page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 297
    Illegal NgoMIV site found at 696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 194
    Illegal SapI.rc site found at 53


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Categories
Parameters
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