Reporter

Part:BBa_K1833003

Designed by: Konstantin Borisov   Group: iGEM15_Pitt   (2015-09-16)


pProt-GFP

This part produces GFP in the presence of sigma-70 E. coli RNA polymerase; however, the promoter contains two sites for a synthetic repressor to bind. Without the presence of this repressor, the part can serve as a strong constitutive promoter. For more information on the repressor, visit http://2015.igem.org/Team:Pitt/Protease/Project.

This part is similar in function to BBa_K1833001, but was synthesized de novo. As such, it contains several unique features. First of all, the sequence for GFP was codon optimized. Secondly, the RBS was optimized for high translational activity using the Salis Lab Ribosome Binding Site calculator (https://salislab.net/software/).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 106
    Illegal AgeI site found at 229
  • 1000
    COMPATIBLE WITH RFC[1000]


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