Project

Part:BBa_K1830017

Designed by: Mathieu Husser   Group: iGEM15_Concordia   (2015-09-18)


Dual docking extracellular scaffold

This part is the main construct cloned by the iGEM Concordia 2015 team. It is the entire DNA sequence that allows for the Induction, expression, targeting and attachment of an extracellular scaffold onto the extracellular membrane of the lactic acid bacteria Lactococcus Lactis. This scaffold contains two different dockerin domain that allow for the specific harboring of cohesin-fused proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6560
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1361
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 301
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None