Project
Part:BBa_K1830016
Designed by: Mathieu Husser Group: iGEM15_Concordia (2015-09-18)
Dual docking extracellular scaffold
This part is the main construct cloned by the iGEM Concordia 2015 team. It is the entire DNA sequence that allows for the Induction, expression, targeting and attachment of an extracellular scaffold onto the extracellular membrane of the lactic acid bacteria Lactococcus Lactis. This scaffold contains two different dockerin domain that allow for the specific harboring of cohesin-fused proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 6560
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1361
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 301
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |