Protein_Domain

Part:BBa_K1830005

Designed by: Mathieu Husser   Group: iGEM15_Concordia   (2015-08-06)

coh2S1

Type II cohesin domain

Usage and Biology

coh2S1 is the type II cohesin domain of the Scaffolding Dockerin Binding protein A (SdbA), part of the Clostridium Thermocellum cellulosome. The cellulosome is an extracellular protein scaffold that displays cellulose-degrading enzymes. The different scaffold component come together through the interaction between cohesin and dockerin domains they contain. These interactions are calcium ion dependant and binding is specific, therefore a type II cohesin will only bind to a type II dockerin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 9
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None