pFig2c: Mating factor alpha responsive promoter
pFig2c is a mating factor alpha responsive promoter, meaning that it is activated by the presence of mating factor alpha. It is endogenous in S. Cerevisiae cells and is followed by the Fig2c gene. The Fig2c gene is a part of the mating pathway in S. Cerevisiae, it contributes to maintaining cell wall integrity when the cells project ("shmoo") towards each other.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 431
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 125
Illegal BsaI.rc site found at 564
iGEM18_NEFU_China designed the experiment and carried out the verification of the promoter. We induced the activation of pFig2c by the α factor we extracted, and the expression of EGFP protein was observed with fluorescence (Fig.1 add with α factor). The negative control (Fig.2, without α factor) showed no fluorescence. [See more details]
So this part is successfully verified.