Protein_Domain
Bar1

Part:BBa_K1829000

Designed by: Nicholas Suen   Group: iGEM15_UCSF   (2015-09-04)


Bar1: Protease that cleaves alpha factor

The Bar1 gene codes for the production of Bar1, a protease that degrades the yeast mating pheromone alpha factor. It has homology to aspartic proteases and has endopeptidase action that cleaves alpha factor between 6-Leu and 7-Lys. Bar1 is secreted into the periplasmic space between the cell membrane and cell wall of MATa S. cerevisiae. It is heavily glycolysated and maintained in the transmembrane space in yeast cells, where it creates a pheromone sinkhole that desensitizes yeast cells to alpha factor signal and sharpens concentration gradients of alpha factor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 340
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None