Part:BBa_K1828902

pH sensitive promoter+XrpA

This part encodes for pH regulated promoter and XrpA protein. pH sensitive promoter is F-ATPase operon promoter of Streptoccoccus Mutans [1]. A promoter is activated at external pH of 5. XrpA encodes for a protein that inhibits natural competence of Streptococcus mutans [2].

References

[1] Sasaki, Y., Nogami, E., Maeda, M., Nakanishi-Matsui, M., & Iwamoto-Kihara, A. (2014). A unique F-type H+-ATPase from Streptococcus mutans: An active H+ pump at acidic pH. Biochemical and biophysical research communications, 443(2), 677-682.

[2] Kaspar, J., Ahn, S. J., Palmer, S. R., Choi, S. C., Stanhope, M. J., & Burne, R. A. (2015). A unique open reading frame within the comX gene of Streptococcus mutans regulates genetic competence and oxidative stress tolerance. Molecular microbiology, 96(3), 463-482.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]