RNA

Part:BBa_K1828901

Designed by: Altynay Abdirakhmanova   Group: iGEM15_NU_Kazakhstan   (2015-09-09)


PnlmAB+sgRNA for VicK

This composite part consists form nlmAB promoter and sequence for sgRNA for VicK. nlmAB promoter is a natural promoter found in Streptococcus mutans. This promoter is upregulated by addition of Competence Stimulating Peptide (CSP). VicK was found to be responsible for lactic acid production and acid tolerance of Streptoccoccus mutans. sgRNA for VicK is designed to specifically target VicK in the genome of S.mutans with deadCas9 that lack endonuclease activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None