Regulatory

Part:BBa_K1826000

Designed by: Jayaprakash S   Group: iGEM15_SVCE_Chennai   (2015-09-18)

Mutant pBAD/araC promoter containing SacI site

This part is an improvement on the biobrick BBa_I0500. It carries a mutation in the form of the introduction of a SacI site in the base number 430. Substitution of C with G introduces a SacI site. We used this for assembling our parts into the pET 24a vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None