Composite

Part:BBa_K1824004

Designed by: Wenbo Xu   Group: iGEM15_XJTLU-CHINA   (2015-09-05)

pBAD + RNA Thermometer FourU

This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer FourU BBa_K1824560 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

XJTLU-FourU.jpg
Figure 1: Possible secondary structure of RNAT FourU.


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None