Composite

Part:BBa_K1824001

Designed by: Wenbo Xu   Group: iGEM15_XJTLU-CHINA   (2015-09-05)

Constitutive Promoter J23119 + RNA Thermometer FourU

This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer FourU BBa_K1824559 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.


XJTLU-FourU.jpg
Figure 1: Possible secondary structure of RNAT FourU.

Characterization

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None