Composite

Part:BBa_K1823003

Designed by: Isyatul Azizah   Group: iGEM15_UB_Indonesia   (2015-09-18)


EDE1C8 + promoter

EDE1C8 is one of broadly neutralize antibody for envelope glycoprotein of Dengue virus. This antibody this came from patients who infected by unknown serotype of Dengue virus,in silico experiment prove that the antibody can bind to all dengue virus envelope glycoprotein. It has promoter and RBS on the upstream site, also has OsmY as a signal peptide that will bring the antibody to periplasm, so, it can be isolate by osmotic shock. You can collect the antibody by purification using Nickel column, it has Histidine (6X).

Note: This part does not have TEV sequence to cleave the targeted antibody and separate the antibody from another prtoein. This scFv antibody has Myc as the linker between the light chain and heavy chain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1166
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1267
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None