Composite

Part:BBa_K1823002

Designed by: Isyatul Azizah   Group: iGEM15_UB_Indonesia   (2015-09-18)


EDE1C8 scFv

EDE1C8 is one of broadly neutralize antibody for envelope glycoprotein of Dengue virus. This antibody this came from patients who infected by unknown serotype of Dengue virus,in silico experiment prove that the antibody can bind to all dengue virus envelope glycoprotein.

To express this antibody in your choice host cell, such as E. coli, add the promoter and RBS on the upstream site. This part also has OsmY as a signal peptide that will bring the antibody to periplasm, so, it can be isolate by osmotic shock.

This scFv antibody has Myc as the linker between the light chain and heavy chain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1104
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1205
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None