Intermediate

Part:BBa_K182003

Designed by: MD. RISAT UL HAQUE, Daniel J. Leirer   Group: iGEM09_Aberdeen_Scotland   (2009-07-20)

Intermediate part consisting of CI promoter followed by tetR, LacI and Tet Operator

This part has been created using the BBa_K182001 and the BBa_k182002. The construct contains a CI regulated promoter with TetR, LacI and Ter promoter attached to it. The BBa_k182001 consists of LacI (RBS+, Term+) and a tet promoter and the BBa_K182002 contains a Lambda CI promoter with a tetR protein containning a ribosomal binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None