Generator

Part:BBa_K1820027

Designed by: Chad Lawrence Nielsen   Group: iGEM15_Utah_State   (2015-09-16)

p2001-native RBS-mCherry-B0015

This part is a reporter sequence designed to produce mCherry fluorescent proteins when the host cell is infected by the P335 species lactococcal phage Φ31.

Usage and Biology

This construct is built from a promoter from the P335 species lactococcal phage Φ31, a native ribosome binding site, an mCherry fluorescent protein that is known to work well in Lactococcus lactis, and a double stop terminator. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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