Intermediate

Part:BBa_K182002

Designed by: Daniel J. Leirer, Md. Risat ul Haque   Group: iGEM09_Aberdeen_Scotland   (2009-07-06)

Intermediate Part consisting of a cI regulated promoter and TetR (RBS+, Term-).

This is an intermediate part using the biobrick BBa_R0051 as the upstream part and BBa_S03518 as the downstream part.

BBa_R0051 is a cI regulated promoter. The promoter has two binding sites for the cI repressor and is repressed when it binds.

BBa_S03518 is a TetR protein with a Ribosome Binding site upstream and an LVA tag attached to its end. TetR binds to TetR regulated promoters such as BBa_R0040. aTc (anhydrotetracycline) binds to TetR causing it to be released from the promoter and reintroducing transcription.

This part comes without a terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/a