Intermediate

Part:BBa_K182001

Designed by: Daniel J. Leirer, Md. Risat ul Haque   Group: iGEM09_Aberdeen_Scotland   (2009-07-06)


Intermediate Part consisting of LacI ( RBS+, Term +) and a Tet operator.

This is an intermediate part using the biobrick BBa_I732820 (RBS, LacI, Term) upstream and the Biobrick BBa_R0040 (TetR repressible promoter) downstream.

It comes with a ribosome binding site upstream of LacI and a Terminator downstream (BBa_B0015) There is no Promoter upstream of LacI, meaning that LacI will not be expressed in this piece.

The TetR repressible promoter BBa_R0040 is downstream of the Terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None