Regulatory

Part:BBa_K1820009

Designed by: Alex Torgesen   Group: iGEM15_Utah_State   (2015-09-16)

CP11

Synthetic promoter created for use in Lactococcus lactis but also shows activity in E. coli, part BBa_K1033221 from the 2013 Uppsala team. Of the three promoters that we chose to use; cp8, cp11, and cp44; this one's activity was between the other two. It does not contain a ribosomal binding site. The paper listed below has more information.

Jensen, Peter Ruhdal and Karin Hammer. "The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." Applied and Environmental Microbiology (1998): 82-87. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/]

To visit the original Uppsala submission of this part, click here: https://parts.igem.org/Part:BBa_K1033221

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None